Anoxia as a treatment against Tetranychus urticae and Spodoptera littoralis

Keywords: Anoxia, CO₂, Reactive Oxygen Species, Spodoptera, Tetranychus


The created anoxic environment has inferred its effect against specific agricultural pests as Tetranychus urticae and Spodoptera littoralis. It was depending on the exposure of both pests with certain stages to 0 ppm O₂ and 5000 ppm CO₂. After anoxia exposure for 8h, no hatchability was detected of both pests’ eggs. Consequently, hatchability percentages were 78.07% and 64.11% for T.urticae and S.littoralis, respectively, after anoxia exposure for 2h. While exposure to 4h to anoxic conditions resulted in a reduction of hatchability percentages which recorded 32.01% and 20.78% for the same arrangement, respectively. Then anoxia effect expanded on the survival percentages of the resulted larvae of T.urticae and S.littoralis, they were 20.36% and 31.78%, respectively, for 2h. While exposure to 4h to anoxic conditions resulted in a decrease in survival percentages which recorded 5.05% and 5.41% for the same arrangement, respectively.                                                                    

Estimated values of LT₅₀ were 5.7,4.1 and 4.77 h, respectively, for eggs, adult males and females of T.urticae, respectively. While, LT50s were 6.09,3.15,4.21, and 6.15 h for anoxia effect of eggs, 2nd and 4th larval stages and pupal stage of S. littoralis, respectively. Anoxic stress led to oxidative stress development. Excessive reactive oxygen species translated in the significant reduction of formation in reactive oxygen scavengers (ROS) in anoxia treatments with LT50 of both T.urticae and S. littoralis which were significantly lower than control. Ascorbate Peroxidase (APX) before exposure to anoxia was higher than after treatments.


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How to Cite
R. ABD EL-WAHAB, “Anoxia as a treatment against Tetranychus urticae and Spodoptera littoralis”, IJCBS, vol. 1, no. 1, Mar. 2020.